Sero-prevalence of Haemonchus contortus infection in sheep by Indirect-ELISA using somatic antigen
نویسندگان
چکیده
منابع مشابه
Immunodiagnosis of Haemonchus contortus infection in sheep by indirect enzyme linked immunosorbent assay (ELISA)
Indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of H. contortus. Plate ELISA was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate...
متن کاملimmunodiagnosis of haemonchus contortus infection in sheep by indirect enzyme linked immunosorbent assay (elisa)
indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of h. contortus. plate elisa was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. indirect plate elisa was able to demonstrate...
متن کاملDetection of anti-Haemonchus contortus antibodies in sheep by dot-ELISA with immunoaffinity purified fraction of ES antigen during prepatency.
Haemonchosis is a very common disease in small ruminants caused by H. contortus, a blood sucking parasite causing anaemia that may be fatal particularly to young animals. Therefore, detection of the infection during prepatent period is important for early treatment. Excretory-Secretory (ES) protein of H. contortus was purified through immunoaffinity chromatography. Dot -ELISA was performed with...
متن کاملImmunological responses of sheep to Haemonchus contortus.
Infections with Haemonchus contortus are a major constraint on ruminant health world-wide. Young lambs are very sensitive to Haemonchus infection. Older lambs and sheep acquire immunity after a continuous or seasonal exposure to the parasite. The mechanisms underlying immunity are still not completely understood. Antibodies, in particular local IgA and IgE, certainly play a role. The role of Ig...
متن کاملEarly detection of Haemonchus contortus infection in sheep using three different faecal occult blood tests
Haemonchus contortus is a blood-sucking parasite causing the presence of faecal occult blood (FOB). The objective was to study three different FOB tests in order to have a new indicator of H. contortus infection in sheep that could be included in the genetic evaluation system as an alternative selection criterion to faecal worm egg count (FEC). A total of 29 Corriedale lambs were experimentally...
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ژورنال
عنوان ژورنال: Journal of Parasitic Diseases
سال: 2014
ISSN: 0971-7196,0975-0703
DOI: 10.1007/s12639-014-0527-2